Pulp tissue obtained from these teeth were smeared in two slides each of which is stained by H and E and quinacrine dihydrochloride for the study of X chromosome and Y chromosome, respectively. A group of 10 (fiveeach of male and female) was examined at various time intervals. Teeth involved in this study were canine and incisors. In this study, a total of 100 cases were analyzed out of which 90 cases were selected from cadavers and 10 cases from patients undergoing treatment. The authors have carried out this study was to determine the period of time after death upto which sex of the individual can be determined, depending on variation of temperature and humidity. Both X and Y chromosomes are found to be present during interphase of the cell cycle. Similar to X chromosome, Y chromosome (F bodies) can also be studied for sex determination of males.

These Barr bodies are present in 40% of females who are considered as chromatin positive and absent in males who are considered as chromatin negative. X chromatin in its inactivated form is present as a mass against the nuclear membrane in females is known as Barr body as it was first named by Barr and Bertem (1949). Apart from this method, it can also be determined by using X and Y chromosomes in the cells which are inactive. Sex of the individual can be determined based on the morphology of canines. J Indian Acad Forensic Med 2004 26:0971-0973ДЇorensic odontology is useful in identification of age and sex of patients. Nirmal Das, Gorea RK, Gargi J, Jai Rup Singh

Chromatin negative nuclei usmle